Journal: ChemMedChem

Article Title: Pyrido[1,2- a ]benzimidazole-Based Agents Active Against Tuberculosis (TB), Multidrug-Resistant (MDR) TB and Extensively Drug-Resistant (XDR) TB

doi: 10.1002/cmdc.201000490

Figure Lengend Snippet: Anti-TB activity of compounds 3a–w, 4–6 against M. tuberculosis strain H37Rv.

Article Snippet: For these compounds, the MIC BD values were the same or slightly higher than those of the corresponding MIC MABA , thereby indicating a cidal effect for this class of derivatives. table ft1 table-wrap mode="anchored" t5 caption a7 Compd MIC MABA [a] [μg mL −1 ] MIC BD [b] [μg mL −1 ] IC 50 Vero cells [c] [μg mL −1 ] Compd MIC MABA [μg mL −1 ] MIC BD [μg mL −1 ] IC 50 Vero cells [μg mL −1 ] 3a 0.5 1.0 8.0 3n >128 nt nt 3b >128 >128 nt [d] 3o 4.0 nt nt 3c >128 >128 nt 3p 4.0 nt nt 3d >128 nt nt 3q 0.5 nt nt 3e >128 nt nt 3r 0.5 1.0 >64 3 f 64 nt nt 3s 8.0 nt nt 3g 8.0 nt nt 3t >128 nt nt 3h 0.25 0.25 >64 3u 4.0 nt nt 3i 2.0 2.0 16 3v 8.0 nt nt 3j 0.5 nt nt 3w >128 nt nt 3k 0.5 nt nt 4 2.0 nt nt 3l 0.25 1.0 8.0 5 1.0 nt nt 3m 0.25 0.5 4.0 6 >128 nt nt INH [e] 0.04 nt nt Open in a separate window [a] The lowest concentration of drug leading to at least a 90% reduction of bacterial growth signal by microplate Alamar Blue assay (MABA); values reported are the average of three individual measurements; [b] The lowest concentration of drug preventing visible bacterial growth in a 5 mL Tween-free Middlebrook broth dilution (BD) culture; values reported are the average of three individual measurements; [c] Cytotoxicity against Vero cells; [d] Not tested; [e] Isoniazid.

Techniques: Activity Assay

Journal: ChemMedChem

Article Title: Pyrido[1,2- a ]benzimidazole-Based Agents Active Against Tuberculosis (TB), Multidrug-Resistant (MDR) TB and Extensively Drug-Resistant (XDR) TB

doi: 10.1002/cmdc.201000490

Figure Lengend Snippet: Comparative anti-TB activity of compounds 3h and selected antitubercular drugs against susceptible, MDR and XDR strains of M. tuberculosis.

Article Snippet: For these compounds, the MIC BD values were the same or slightly higher than those of the corresponding MIC MABA , thereby indicating a cidal effect for this class of derivatives. table ft1 table-wrap mode="anchored" t5 caption a7 Compd MIC MABA [a] [μg mL −1 ] MIC BD [b] [μg mL −1 ] IC 50 Vero cells [c] [μg mL −1 ] Compd MIC MABA [μg mL −1 ] MIC BD [μg mL −1 ] IC 50 Vero cells [μg mL −1 ] 3a 0.5 1.0 8.0 3n >128 nt nt 3b >128 >128 nt [d] 3o 4.0 nt nt 3c >128 >128 nt 3p 4.0 nt nt 3d >128 nt nt 3q 0.5 nt nt 3e >128 nt nt 3r 0.5 1.0 >64 3 f 64 nt nt 3s 8.0 nt nt 3g 8.0 nt nt 3t >128 nt nt 3h 0.25 0.25 >64 3u 4.0 nt nt 3i 2.0 2.0 16 3v 8.0 nt nt 3j 0.5 nt nt 3w >128 nt nt 3k 0.5 nt nt 4 2.0 nt nt 3l 0.25 1.0 8.0 5 1.0 nt nt 3m 0.25 0.5 4.0 6 >128 nt nt INH [e] 0.04 nt nt Open in a separate window [a] The lowest concentration of drug leading to at least a 90% reduction of bacterial growth signal by microplate Alamar Blue assay (MABA); values reported are the average of three individual measurements; [b] The lowest concentration of drug preventing visible bacterial growth in a 5 mL Tween-free Middlebrook broth dilution (BD) culture; values reported are the average of three individual measurements; [c] Cytotoxicity against Vero cells; [d] Not tested; [e] Isoniazid.

Techniques: Activity Assay

Journal: BMC Cancer

Article Title: In vitro antineoplastic effects of auranofin in canine lymphoma cells

doi: 10.1186/s12885-018-4450-2

Figure Lengend Snippet: Auranofin exhibits dose-dependent growth inhibitory effects in canine and human lymphoid tumor cells. 4 canine ( a ) and 2 human ( b ) lymphoid tumor cell lines were incubated with AF for 72 h, followed by determination of relative viable cell number using a bioreductive fluorometric assay. Curves represent means of three independent experiments, and error bars indicate SEM

Article Snippet: Relative viable cell number was determined using a bioreductive fluorometric assay (Alamar Blue, Promega, Madison, WI) according to manufacturer directions, using a Synergy HT plate reader (Bio-Tek, Winooski, VT) and expressed as a percentage of control-treated cells.

Techniques: Incubation

Journal: BMC Cancer

Article Title: In vitro antineoplastic effects of auranofin in canine lymphoma cells

doi: 10.1186/s12885-018-4450-2

Figure Lengend Snippet: Auranofin synergizes with DOX and CCNU in canine lymphoma cells. Canine lymphoma cell lines 1771 and OSW were incubated with AF, DOX, CCNU, AF + DOX or AF+ CCNU for 72 h, followed by determination of relative viable cell number using a bioreductive fluorometric assay. Bars represent means of three independent experiments, and error bars indicate SD. The significance of differences between groups was analyzed by one-way ANOVA on ranks with Tukey’s post hoc test. ** p < 0.01, * p < 0.05

Article Snippet: Relative viable cell number was determined using a bioreductive fluorometric assay (Alamar Blue, Promega, Madison, WI) according to manufacturer directions, using a Synergy HT plate reader (Bio-Tek, Winooski, VT) and expressed as a percentage of control-treated cells.

Techniques: Incubation

Journal: BMC Cancer

Article Title: In vitro antineoplastic effects of auranofin in canine lymphoma cells

doi: 10.1186/s12885-018-4450-2

Figure Lengend Snippet: Auranofin inhibits the growth of canine primary lymphoma cells in a dose-dependent manner. Four canine primary B-cell lymphoma cultures were incubated with AF for 72 h, followed by determination of relative viable cell number using a bioreductive fluorometric assay. Individual colors represent cells derived from individual dogs. Curves represent means of three independent experiments, and error bars indicate SD

Article Snippet: Relative viable cell number was determined using a bioreductive fluorometric assay (Alamar Blue, Promega, Madison, WI) according to manufacturer directions, using a Synergy HT plate reader (Bio-Tek, Winooski, VT) and expressed as a percentage of control-treated cells.

Techniques: Incubation, Derivative Assay